Journal: Frontiers in Plant Science

Article Title: Peroxynitrite-mediated tyrosine nitration modulates β-1,3-glucanase activity and potato defense against Phytophthora infestans

doi: 10.3389/fpls.2026.1796014

Figure Lengend Snippet: Tyrosine nitration pattern in potato leaves challenge-inoculated with Phytophthora infestans at 4 and 48 h post-inoculation (hpi) in the resistant genotype. Representative 2D electrophoresis (pH 4–7 for the first dimension) of potato-avr P. infestans stained with CBB G-250 and representative immunoblots probed with a polyclonal antibody against nitrotyrosine diluted at 1:1000. Molecular-mass standards (kDa) are indicated on the left. Arrowheads indicate all the immunoreactive spots, the symbols (s1–s45) refer to the proteins listed in Table 1 . The quantitative results for protein Tyr nitration were calculated using PDQuest 2-D Analysis Software (Bio-Rad), and the data were presented relative to the control sample, with the average set to 0 (control – mock-inoculated genotype). Asterisks indicate values that differ significantly from the control at *p < 0.05.

Article Snippet: 3-nitrotyrosine (3-NT) levels were quantified using the OxiSelectTM Nitrotyrosine ELISA Kit (Cell Biolabs) according to the manufacturer’s instructions.

Techniques: Nitration, Two-Dimensional Gel Electrophoresis, Staining, Western Blot, Software, Control

Journal: Frontiers in Plant Science

Article Title: Peroxynitrite-mediated tyrosine nitration modulates β-1,3-glucanase activity and potato defense against Phytophthora infestans

doi: 10.3389/fpls.2026.1796014

Figure Lengend Snippet: Tyrosine nitration pattern in potato leaves challenge-inoculated with Phytophthora infestans at 4 and 48 h post-inoculation (hpi) in the susceptible genotype. Representative 2D electrophoresis (pH 4–7 for the first dimension) of potato-vr P. infestans stained with CBB G-250 and representative immunoblots probed with a polyclonal antibody against nitrotyrosine diluted at 1:1000. Molecular-mass standards (kDa) are indicated on the left. Arrowheads indicate all the immunoreactive spots, the symbols (s1–s32) refer to the proteins listed in Table 1 . The quantitative results for protein Tyr nitration were calculated using PDQuest 2-D Analysis Software (Bio-Rad), and the data were presented relative to the control sample, with the average set to 0 (control – mock-inoculated genotype). Asterisks indicate values that differ significantly from the control at *p < 0.05.

Article Snippet: 3-nitrotyrosine (3-NT) levels were quantified using the OxiSelectTM Nitrotyrosine ELISA Kit (Cell Biolabs) according to the manufacturer’s instructions.

Techniques: Nitration, Two-Dimensional Gel Electrophoresis, Staining, Western Blot, Software, Control